Karuppiah Valliappan

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Questions related from Karuppiah Valliappan

Seeking Detailed Protocol for Rabbit Immunization with Soluble Protein?

I am looking for a comprehensive protocol for immunizing rabbits with a soluble protein. Specifically, I need detailed information on the following aspects: Rabbit Age and Preparation: What is the...

29 August 2024 7,193 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

23 July 2024 9,556 2 View

I am facing problem in the subculturing of hybridoma clone from HAT medium to HT medium?

I am standardising the methodology for hybridoma production. I did fusion with PEG and cultured in HAT supplemented DMEM+10%FBS. after five days I found several cluster of cells in HAT...

07 February 2022 4,506 10 View

How to estimate the auxin, cytokinin gibberellin and Betain in the liquid seaweed extract ?

Dear Experts, Kindly suggest me a best protocol to estimate the auxin, cytokinin gibberellin and Betain in the liquid seaweed extract.

18 April 2018 2,457 5 View

How to estimate the potassium sulphate in the organic fertilizers ?

Dear Experts, kindly suggest me a best method to estimate the percentage of potassium sulphate in the organic fertilizers.

10 April 2018 1,922 12 View

Epperndorf biospectrophotometer vs. Nanodrop?

Can someone tell me if epperndorf biospectrophotometer with micro cuvette is really better than Nanodrop in nucleic acids quantification, even for small concentrations?

17 March 2016 9,670 5 View