Diclofenac before encapsulation and after encapsulation gives you amount of drug encapsulated.

Drug release can be calculated by allowing your system to expose solvent that favours diclofenac to be released ( liquid-liquid extraction).

Calculated diclofenac on time scale, to determine the behavior of release from your encapsulated system.

Hi,

They use buffer pH = 7.4 due to diclofenac is slightly soluble in water under acidic pH, moreover the use of a solvent can cause interferences by solubilization of other components. Further, the encapsulation percent can be calculated directly or indirectly. For direct method, I suggest to dissolve your dry final product by magnetic stirrer or utraturrax. Furthermore, for indirect method you must take an aliquot of your suspension, ultracentrifugate and reading the sobrenadant. For release study you can use the dialysis method.

Dear Samar Khattab,

Thin film hydration method is suitable for the hydrophilic and hydrophobic drug. However, since your drug is soluble in the water (hydrophilic), so you can try to dissolve your drug in the hydration solvent to encapsulate your drug easily.

To choose the correct solvent for calibration curve, it will depend on the release medium. You have to choose a similar medium.

Good luck!

CV Chandra Sekhar Reddy

thank you for reply

Yhors Ciro

i will try the direct method for the encapsulation percent

thanks

Raditya Iswandana

I will study the release in pH=7.4 so calibration curve done in pH7.4

THANKS