I am developing a method for enantioseparation of fluoxetine, atenolol and tramadol using Chiralpak AD-H and Chiralpak IA. Is there any reliable, published method you can recommend? I used a published method for Fluoxetine using chiralpak AD-H with mobile phase n-hexane/2-propanol/diethylamine (98/2/0.1%) and could not obtain any resolution even though the authors claimed a resolution of 1.75. Are there any other suggestions or I should use reversed phase hplc with Chirobiotic V column?
in chiral chromatography your choices of mobile phase are rather limited. If you have not obtained resolution, better change the column. BTW: are you sure that the additive is Et2NH?
Yes, they used diethyl amine in the paper. I have tried using Chiralpak IA, AD-H and Chiralcel OJ-H. I have used only n-hexane and 2-propanol in varying ratios.
http://link.springer.com/article/10.1631/jzus.2007.B0056
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