Hello everyone,
due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ between mice. As the ELISPOT result is stated in spots per 1E6 cells, can I adjust this based on the actual cell number?
As an example, instead of 1E6 cells, the well only contained 5E5 cells. If I cound 200 spots, can I state the number of spots as it were if I had added the correct cell number? So here, instead of 200 Spots / 5E5 cells i would plot it as 400 spots per 1E6?
A repeat would of course be most accurate, but I was out of plates and peptides. The cells are frozen, so I can repeat the assay in the new year sas well.
looking forward to any help you can give me.
Provided the cell treatments and incubation techniques are not concentration dependent I would think it would be ok to equate 200 spots per 5 x 105 cells with
400 spots per 106 cells .