Erik S Haefner

3 Questions 6 Answers 0 Followers

Questions related from Erik S Haefner

ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

16 December 2020 9,542 1 View

What is the AnnexinV-/7-AAD+ population? Transfected cells show up in this population. What are they?

I have been performing a Annexin V/7-AAD timecourse of tumor cells transfected with different amounts of RNA. In one group getting a high dose of a virus derived RNA encoding GFP, I see the entire...

11 December 2017 5,623 2 View

What is a standard or common Target-to-effector cell ratio for T cells in an assay?

I am looking for standard or common ratios of Target cells to T cells in vitro. I'm currently thinking of 1:1-1:60 but this is arbitrary. Is there a certain range one should cover or do you just...

07 May 2013 7,855 5 View