Transwell migration assay - cells dying !?

Hi all, using a transwell assay to test migratory function of cytokines, Costar transwells 5 micrometer, 24 well, according to publications and protocols 100 ul in top compartment with 1x10*6...

09 October 2011 4,678 2 View

MSP stadardization problem

Read 1 answer by scientists to the question asked by Axel Hueber on Oct 8, 2009

09 October 2009 6,627 0 View

protein science

Read 2 answers by scientists to the question asked by Axel Hueber on Oct 8, 2009

09 October 2009 7,156 1 View

Caspase 3 - Please Help!

Read 4 answers by scientists to the question asked by Axel Hueber on Sep 18, 2009

08 September 2009 1,153 2 View

nonspecific bands in CO-IP

Read 2 answers by scientists with 1 recommendation from their colleagues to the question asked by Axel Hueber on Sep 18, 2009

08 September 2009 6,525 0 View

SINGALING PATHWAY IN A CELL

Read 1 answer by scientists with 1 recommendation from their colleagues to the question asked by Axel Hueber on Aug 1, 2009

07 August 2009 3,593 0 View

i need help w HMEC culturing

i'm culturing my HMEC in gelatin -coated flasks (by me) using MCDB medium and EGF, heparin and FBS (10%) and the cells did not attach... what...

07 August 2009 6,047 0 View

copy number determination: sauthern blott vs real time pcr

Read 2 answers by scientists to the question asked by Axel Hueber on Aug 25, 2009

07 August 2009 156 0 View

Sequencespace

Read 1 answer by scientists to the question asked by Axel Hueber on Aug 25, 2009

07 August 2009 1,030 0 View

hi

07 August 2009 4,848 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View