Dear Afnan!

Please You look at the article:

Article Epithelial-Mesenchymal Transitions in Development and Disease

Figure 5. EMT Signaling Pathways

EGF, epidermal growth factor;

This EMT is induced by epidermal growth factor (EGF) and involves the activation of metalloproteases and of ILK and ERK kinases (Ahmed et al., 2006).

I find some response to your question in a canadian work from Nino Lyette which found in 1998 thatThis suggests that The activation induced by EGF ("epidermal growth factor" or growth factor epidermal) is essential so that HCB can exert its mitogenic power against epithelial breast cells. Than EGF has an indirect action?

Hello Afnan Saleem

Also please refer to the research article below:

Summary:

A seamless plasticity exists among cells shifting between epithelial and mesenchymal phenotypes during early development and again later, in adult tissues, following wound repair or organ remodeling in response to injury. Fsp1, a gene encoding a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo. In the current study, we identified several cytokines that induce Fsp1 in cultured epithelial cells. A combination of these factors, however, was most efficacious at completing the process of EMT. The optimal combination identified were two of the cytokines classically associated with fibrosis, i.e., transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF). To confirm that it was the induction of Fsp1 by these cytokines mediating EMT, we used antisense oligomers to block Fsp1 production and subsequently measured cell motility and markers of EMT phenotype. The antisense oligomers suppressed Fsp1 expression and epithelial transformation; therefore, we conclude that the appearance of Fsp1 is an important early event in the pathway toward EMT.

Article Early role of Fsp1 in epithelial-mesenchymal transformation

Best.

It's a thesis in french which is freely available in google.

Dear Alexandr Chernov

We tried EGF alone & in combination with insulin to undergo transformation for 72 hrs. But, we did not observe any change in morphology.