could you please provide me with some articles that done experiments on mice by using plain Gelatin? I want to study the effect of plain gelatin on mice
Hello How to perform community network analysis using GROMACS trajectory Thank you so much
20 December 2022 753 0 View
Dear all, The complex I am trying to simulate has protein ( 10 chains), DNA, and RNA I ran the simulation for 2 us so far but the biological event I want to observe requires a very long MD...
01 June 2021 1,556 1 View
Hi I would like to MD simulate a CRISPR cryo-EM- structure. But only a small region of the R-loop was observed. I want to simulate the R-loop formation. How to model the missing part of the...
21 February 2021 6,025 0 View
Hi all I used the following script to run the simulation but for some reason, I was not able to assign GPU to do the PME calculation? How to do that? The size of the system is huge (600.000...
14 January 2021 7,654 1 View
Hi My system I am trying to simulate has 600.000 atoms. I want to simulate for 1 us I am using a supercomputer to run the simulation with tesla K80 GPUs. I am using 1 node and a total of 28 core...
08 January 2021 4,903 1 View
How to add a new termini database entries in amber FF for phosphorylated at 5'- and 3'-termini of DNA? Pdb2gmx is not able to covert pdb to gro files could you please help me?
22 September 2020 8,562 7 View
hello Is polyethlene glycol ( 5000 D) soluble in aceton and ethanol?
12 November 2018 2,216 1 View
PEG does not have chromophore do you know how to add a chromphore to make it detectable by UV detector? what is the chemistry behind that can anyone help me with that thank you
10 November 2018 4,743 1 View
10 November 2018 9,599 1 View
Hello The standard time in our lab is 4 hrs for MCF-7 but the goal of my experiment is to study the effect of assay? The standard time in our lab is 4 hrs for MCF-7 but the goal of my...
03 April 2018 3,863 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View