Hi Amnah!

I think 2 hrs incubation of MTT is still good enough to form farmazan crystals.

Hi, see the answers of following question

https://www.researchgate.net/post/Can_incubation_time_influence_in_cell_assays

Hi Amnah,

in my lab we use cell counting kit-8 (CCK8) from Dojindo to for assessing cell viability, proliferation and cytotoxicity. I've used incubation times of 1 and 2 hours at 37C and have seen good reproducibility. This may be a good alternative if you require shorter incubation times.

http://www.dojindo.eu.com/store/p/456-Cell-Counting-Kit-8.aspx

hope this helps,

Dan

Hi, 2 hours is suitable.

Best