Modelling missing nt in R-loop?

22 February 2021 0 6K Report

I would like to MD simulate a CRISPR cryo-EM- structure. But only a small region of the R-loop was observed. I want to simulate the R-loop formation. How to model the missing part of the R-loop? I have tried to align the structure against similar structures that have a complete R-loop structure but it didn't work

Any advice?

I really appreciate all your help

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