Bree Barand

5 Questions 3 Answers 0 Followers

Questions related from Bree Barand

Effect of cell size on gradient centrifugation?

Hi y'all, does anyone know if animal cells increase in size as they age or do they simply multiply? I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it...

08 June 2024 4,028 1 View

Recommendation for pretreatment of pup mouse liver for FFPE tissue?

Can anyone recommend fixation techniques for neonatal mouse livers? We usually fix our adult-derived organ sections (about 0,5-1cm in length and 3-4mm in thickness) in 4% PFA for 48 hours at RT,...

09 May 2024 8,310 1 View

Toe clipping demonstration?

Can anyone recommend toe clipping demonstration videos od resources with images? I have to toe clip P1 mice and no one in my lab has ever done it before. A visual aid would be great. Thanks in...

02 December 2023 7,519 0 View

How important is cell-purity in RNA-sequencing? How is single-cell RNA-Seq preferable to bulk RNA-Seq?

Hi everyone, please bear with me, because I am a complete beginner with regard to any form of bioinformatics and I am trying to understand the best approach to my experiment. I am currently...

15 March 2023 6,060 1 View

Picro Sirius Red Staining Background?

We´ve been using the Picro Sirius Red staining for several years now (done in out core facility) to show fibrosis development. However suddenly our stainings, which usually had a yellow background...

14 October 2022 2,426 4 View