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Questions related from Bree Barand
Hi! In my lab we work with density gradients (mostly Nycodenz) a lot to isolate mostly liver NPCs. But I´ve had quite a bit of trouble to understand how exactly that works. While I get that the...
18 October 2024 755 0 View
Since we work mostly with genetic models, we usually genotype our mice after ear punching them for further breeding purposes. Recently our breeding has taken up qutie a bit resulting in a high...
11 September 2024 5,881 1 View
Hi y´all, I work with pups and, as to increase the chance of pregnancy, breed the animals for one week at a time. That leaves me with a pretty broad time frame with regard to when pups might be...
29 July 2024 2,713 0 View
I work with pups at P3 and P9 and usually remove all animals from the female to harvest the organs. I am aware that there is a postpartum estrus (which I, unfortunately, cannot use due to the...
28 July 2024 1,045 1 View
Hi y'all, does anyone know if animal cells increase in size as they age or do they simply multiply? I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it...
08 June 2024 4,155 1 View
Can anyone recommend fixation techniques for neonatal mouse livers? We usually fix our adult-derived organ sections (about 0,5-1cm in length and 3-4mm in thickness) in 4% PFA for 48 hours at RT,...
09 May 2024 8,389 1 View
Can anyone recommend toe clipping demonstration videos od resources with images? I have to toe clip P1 mice and no one in my lab has ever done it before. A visual aid would be great. Thanks in...
02 December 2023 7,604 0 View
Hi everyone, I´m planning on performing experiments with young pups (P2, P4, P6). Does anyone have experimence with when their mothers can be rebred after the pups have been removed and whether...
02 December 2023 1,101 0 View
Hi everyone, please bear with me, because I am a complete beginner with regard to any form of bioinformatics and I am trying to understand the best approach to my experiment. I am currently...
15 March 2023 6,125 1 View
We´ve been using the Picro Sirius Red staining for several years now (done in out core facility) to show fibrosis development. However suddenly our stainings, which usually had a yellow background...
14 October 2022 2,501 4 View
Out laboratory used Dako´s AEC substrate up until two months ago. Since it wasn´t available in our region, we instead received the DAB products. In all cryo liver staining attemps however, we´ve...
14 May 2022 5,685 0 View
