Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

What is the best lighting design for conservation?

In the museums, the sensitive organic materials need to be displayed under 50 lux or less and aways from any UV or IR. LEDs still have a little amount of UV. What is the better option? Is it...

20 January 2021 5,965 3 View

Cryopreservation of cancer cell lines @-80?

07 January 2021 5,410 8 View

Can a researcher depends the conventional PCR as molecular based technique for quantification of STAT3in stead of real time PCR in FFPE tissue ?

The FFPE tissues are relatively difficult to extract the RNA from so, how one can use reverse transcription based PCR

10 December 2020 4,398 6 View

Can anyone help me to solve this problem?

when i did time history anlysis by using sap 2000 , the program showed the first two step only at .02 and .04 . can anyone help me to solve this problem?

27 November 2020 3,810 2 View

Analytical solution of stresses distribution due to tunneling in expansive soil?

I want to know how can I do an analytical solution for stresses distribution due to tunnel excavation in an expansive soil to compare with a numerical simulation. and which method I can use?

22 September 2020 7,602 2 View

Meshing problem in abaqus?

Can any one help me in meshing in ABAQUS, please?

09 September 2020 7,957 7 View

Why halogen species lower the flame temperature?

Chlorine, a component of PVC polymers, acts like a halogen-based flame retardant agent in the event of fire.

07 September 2020 5,450 6 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

How sure are you, that after TUR-B for NMIBC, to have enough muscle tissue for correct histopathological examination?

Deep resection for correct histopathological analysis in TUR-B can be challenging for the surgeon with a low to mid-experience level. This leads to inadequate analysis, possibly postponing radical...

11 April 2020 5,713 3 View

What is awareness level of physical therapy treatment in women with urinary incontinence?

hello i am looking for recent cross sectional studies on physiotherapy treatment awareness in UI. also i am student of final year of dpt so i want a standard questionnaire regarding my research...

04 May 2019 4,950 4 View

Is trace element required for curing disease?

Trace elements are required, but these trace elements are also related to cancer, Various malignant neoplasms of different organs are often seen in idiopathic hemochromatosis, but liver cancer is...

04 May 2019 3,480 3 View

Is there any publically available clinical dataset for cystoscopy images?

I want to work on images/videos of human bladder examination using endoscopes particularly for texture reconstruction. Any lead would be really helpful.

16 January 2019 2,759 2 View

Can we use RPMI 1640 medium for the growth of C57BL/6 Mouse Primary Bladder Epithelial Cells?

Because I want to grow the Primary Bladder Epithelial Cells of C57BL/6 Mouse for my experiment..

31 December 2018 1,957 0 View

Is there any publically available clinical dataset for cystoscopy images?

I want to work on images/videos of human bladder examination using endoscopes particularly for texture reconstruction. Any lead would be really helpful.

31 December 2018 1,687 1 View

HT1376 cell line doesn't seem to be growing properly. It is taking around a week and is still not 80% confluent. Can anyone help me?

I am using DMEM media with 10% FBS and 1% Pen/Strp. It takes a long time for trypsinisation also. I grew the cells for a week and the cell count gave a count of 0.6 million cells only. I have...

11 December 2018 8,652 4 View

HT1376 cell line doesn't seem to be growing properly. It is taking around a week and is still not 80% confluent. Can anyone help me?

I am using DMEM media with 10% FBS and 1% Pen/Strp. It takes a long time for trypsinisation also. I grew the cells for a week and the cell count gave a count of 0.6 million cells only. I have...

09 December 2018 8,596 3 View

How does a bony fish use the swim bladder?

Bony fish

10 November 2018 4,093 16 View