What is the maximum number of personal articles that an author should cite in his or her new article?

It is a good practice for an author to cite some of his or her published work. However, this practice has been abused to gain citations.

21 December 2020 1,385 8 View

I'm looking for a good pH 13 buffer recipe for hydrolysis experiment?

I did try phosphate using Na2HPO4.7H20 and NaOH but buffering capacity was very low especially upon dilution.

09 August 2020 9,692 4 View

I am faced with obtaining SVD and pseudo inverse of this (3x2) matrix A?

Please can someone help with the SVD of the matrix A and send me the pictures to my email? [email protected] Please note, I have tried the possible ways and I am still working on...

11 August 2019 8,468 3 View

Doubly periodic conditions (two-directions) for turbulent rectangular channel flow using LES in ANSYS Fluent?

I need a periodic BC where mass flow rate is specified to model fully developed flow, and another periodic BC with no pressure drop. However, once I define the mass flow rate for one periodic BC,...

18 November 2018 4,845 7 View

I'm planning a glucose uptake assay.

I'm planning a glucose uptake assay with adult mesenchymal stem cells and possibly rat neural stem cells for my undergraduate thesis. i'd appreciate some recommendations for uptake kits and...

18 July 2017 2,222 1 View

In soil contaminant transport, what are other modes of transport apart from advection, sorption and degradation?

03 February 2017 1,444 4 View

How do I pool RNA samples for sequencing?

We have done a disease model mouse experiment, for which there were two treatments, let's call them A and B, yes/no. So we have 4 strata, and we have 9+/-1 samples per strata. We aim to look...

13 September 2015 7,901 12 View

What pH of refolding buffer will be suitable for refolding (renaturing) a solubilized Inclusion Body of protein with isoelectric point (PI) 6.2?

I am trying to solubilize and refold an inclusion body of NKG2D with theoretical isoelectric point (PI) value of 6.2. I usually purify my solubilized protein with Nickel column (Elution buffer pH...

15 December 2013 4,955 4 View

Can any one help me with a suitable protocol for double digestion of a vector(pET22b) and an insert (DNA) with BAHM1 and NOT1 restriction enzymes?

I want to insert my recombinant DNA of about 1650bp into pET22b which is about 5500bp. The resultant product from the ligation will be used for E. coli transformation. I seem not to get a...

21 September 2013 5,706 12 View

Suitable DNA codon for the linker (G4S)3 for expression in E. coli?

I want to link two proteins with the linker (G4S)3. The linked (fused) proteins will then be expressed in E. coli. I will therefore be grateful if anybody could recommend a suitable DNA codon for...

07 March 2013 3,645 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View