Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Ferret PBMC Fc block?

Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...

18 January 2021 4,504 5 View

Platelet Counting on Flow Cytometry?

Hi, Does anyone have a method for counting platelets on flow cytometry (cytometer=Cytoflex) without the need of using TRUcount beads or reference beads? Is it possible or do I need to purchase...

23 September 2020 3,509 1 View

What is the best bibliographic output style for preparing a CV?

I am looking for an output style in Endnote to list publications in one's CV. These would have to be be listed in reverese chronological order. Recommendations and opinions welcome.

23 March 2020 1,228 4 View

Anyone using stereo trail cameras (camera traps) linked to gather morphometric data from cryptic species?

Looking to connect with any research, papers or groups using remotely triggered stereo cameras to create 3 dimensional pictures of wildlife and gather data on cryptic species. Wanting to set up a...

01 February 2020 940 3 View

Finding EA(energy absorption)?

Hello every one, I am new to abaqus. How do I find energy indicators like, EA(energy absorption), Mean Crush Force (MCF) or Crush Force Efficiency (CFE) in a result section for a buckling process...

05 May 2019 5,193 3 View

What am I doing wrong - extracting a sapogenin for NMR?

I use hydrochloric acid to release a steroidal sapogenin from its saponin, then neutralise it with sodium bicarbonate. I am confident the hydrolysis works because the mixture no longer produces a...

03 June 2018 3,814 3 View

Wrist Laxity carpal kinematics: Anyone know where I can find this conference abstract?

My library cannot locate: Effects of wrist laxity on three-dimensional carpal kinematics. J Orthop Res Conf. 2017,35(Supplement 1). by Stoop, Best, Pichora, Kamal, Rainbow Any assistance in...

24 April 2018 2,543 3 View

Unpuried PCR fine, Purified PCR smears?

Ran two Long Amp PCRs, one using un-purified template and the other a purified template. Un-purifed produces a PCR band and the purified doesn't. Negative control has no band or smears in it....

19 February 2018 2,456 4 View

How to detect chemical induced apoptosis?

18 September 2017 9,312 4 View

No title

03 March 2021 5,360 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View