I am trying to clone a gene of interest and want to it mutated. E.coli strains of Rosetta and Rosetta-gami-pLysS seems not working.
Can anyone suggest a strain of E.coli that can achieve this.
03 April 2017 1,106 3 View
Need to understand how beta amylase is been regulated in plants, their relationship with free and bound beta amylase
05 June 2015 2,362 0 View
I am working on Native electrophoresis but need to improvise in order to get a molecular weight standard as the standard with me is faulty and it takes weeks to order a new one. How do I get...
01 February 2015 1,085 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...
03 March 2021 9,692 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...
25 February 2021 7,603 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...
24 February 2021 7,379 4 View
Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...
19 February 2021 2,648 23 View
I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...
18 February 2021 8,198 6 View