I am trying to clone a gene of interest and want to it mutated. E.coli strains of Rosetta and Rosetta-gami-pLysS seems not working.
Can anyone suggest a strain of E.coli that can achieve this.
Can anyone kindly help with the protocol(s) for 16S rRNA sequencing, cloning and expression of beta amylase gene in S. cerevisae. How to identify a microorganism using the 16S rRNA sequencing.
03 April 2017 1,230 3 View
Need to understand how beta amylase is been regulated in plants, their relationship with free and bound beta amylase
05 June 2015 2,487 0 View
I am working on Native electrophoresis but need to improvise in order to get a molecular weight standard as the standard with me is faulty and it takes weeks to order a new one. How do I get...
01 February 2015 1,243 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...
31 July 2024 5,309 7 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...
23 July 2024 5,703 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
14 July 2024 3,570 5 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View