Frank Abimbola Ogundolie

4 Questions 19 Answers 0 Followers

Questions related from Frank Abimbola Ogundolie

E.coli strain that generate random mutation during cloned gene?

I am trying to clone a gene of interest and want to it mutated. E.coli strains of Rosetta and Rosetta-gami-pLysS seems not working. Can anyone suggest a strain of E.coli that can achieve this.

12 December 2017 9,741 0 View

Protocol for Gene Cloning and Expression?

Can anyone kindly help with the protocol(s) for 16S rRNA sequencing, cloning and expression of beta amylase gene in S. cerevisae. How to identify a microorganism using the 16S rRNA sequencing.

04 April 2017 1,183 3 View

I need to understand the understand the role of thiols in regulation and control of in vivo activity. notably beta amylase?

Need to understand how beta amylase is been regulated in plants, their relationship with free and bound beta amylase

06 June 2015 2,442 0 View

Is it logical to improvise Molecular weight standard for Native electrophoresis without having to order a standard marker? How do I go by this?

I am working on Native electrophoresis but need to improvise in order to get a molecular weight standard as the standard with me is faulty and it takes weeks to order a new one. How do I get...

02 February 2015 1,180 3 View