I m using ECL kit for western blot is it necessary to store at 4'c and any impact on function if i store at 4'c, i m using BIO-RAD ECL substrate kit, i m using Nitro celalulose membrane for transfer, i m facing problems so
In general ECL is stored at 4o C, but when you use it should be at room temperature. A good practice will be to take out of the fridge the ECL solutions when you start the final wash. Otherwise you could pipette 300ul of each solution in an eppendorf and use this on the membrane after the final wash. The small volume reach easily room temp.
Thank you sir becoz i m facing some problem at the final step of the detection
It also may caused by which type of ECL were you using, I suggest to mix Pico and Dura ECL kits (1:1) in room temperature, to adjust better imaging of bands. I think storing ECL in the 4C is just for long term using.
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