Sunilgowda S N

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ECL ki storage conditions at RT or 4'c ? any impact on function?

I m using ECL kit for western blot is it necessary to store at 4'c and any impact on function if i store at 4'c, i m using BIO-RAD ECL substrate kit, i m using Nitro celalulose membrane for...

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Anybody know how to prepare sample for HPLC and LC-MS?

I m working on cell culture and i need to analyse the set of proteins and their different additional groups at diffrent conditions and i dont know how to prepare sample for HPLC and LC-MS , i want...

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How to differentiate dead cells and contamination?

i am using A549 cells its growing very fast and i am facing problem in differentiating cells and contamination but it is attending confluence very fast, i am using T25 flask. I can see dead cells,...

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