How should i properly grow EAEC or STEC in LB enriched medium with acetate or formate, before IL-8 infection Assay in T84 cells? Should i buffer the medium with HEPES 100mM (pH 8) or MOPS 100mM (pH 6.7)?
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel
28 July 2024 2,607 2 View
Dear researchers, In a systematic review (not a meta-analysis) compiling studies on various diagnostic systems against a specific pathogen. The most relevant parameters evaluated are the type of...
03 July 2024 9,069 3 View
What is the current usage and patronage of parks and greenspaces by older adults (aged 65+) in Salford? How does the frequency of older adults' visits to parks and greenspaces in Salford relate to...
22 June 2024 7,616 1 View
A question to see who can answer it. If the red and infrared bands of the satellites are...
16 June 2024 2,249 1 View
Hi there! We are trying to use the Concentric Circles Plugin on ImageJ to determine signal intensity in neurosphere cryosections after immunocytochemistry/confocal analysis. We determined the...
12 June 2024 7,332 0 View
I am a first-time researcher in the field of nursing and i would like to conduct research about knowledge and attitude towards prevention and spread of tuberculosis.
11 June 2024 3,821 2 View
Dear colleagues, I'm planning to start neuron mouse co-culture with astrocyte and microglia but I heard that it's difficult to keep it up. Any advices?
27 May 2024 4,905 3 View
I'm having trouble packaging lentivirus in HEK293T using pCMV, pMDG, an shRNA and polyfect. I normally seed 1.5 x 106 HEK293T cells in 60 mm on Day 0, which translates to about 60 to 70%...
20 May 2024 9,067 1 View
I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...
14 May 2024 2,291 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View