Hello everyone!

I am working with DsRed and GFP expressing mice and noticed that there are different cells with different expression levels of DsRed or GFP in a same tissue. For instance, I checked the bone marrow of these mice and noticed that there is a population that express higher levels of GFP or DsRed and there is another population that is dimmer and express lower levels of GFP or DsRed. Interestingly, the size of these cells is also different in the forward and side scatter of FACS dot plots. Should mention that the viability was over 90%, so these cells were not dead. Does anyone have the same experience and possibly know what is going on with these mice??

Thanks !