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Questions related from Mahsa Shirin
I need to show the co-localization of my protein of interest in the membrane in HL60 round and small cells. So I took z-stack images to show the surface expression of the membrane marker that I...
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I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...
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Hello, Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?
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Hello, I am doing co-immunoprecipitation to study the interaction of my protein of interest. The protein concentration after performing the Bradford assay for input samples is deficient. Does...
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