Mahsa Shirin

4 Questions 5 Answers 0 Followers

Questions related from Mahsa Shirin

How can I present the data of Z stack images taken in confocal microscope for a paper or presentation?

I need to show the co-localization of my protein of interest in the membrane in HL60 round and small cells. So I took z-stack images to show the surface expression of the membrane marker that I...

25 November 2024 3,757 3 View

Why there is no endogenous expression of my protein?

I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...

03 May 2023 3,607 4 View

DSP (cross-linking reagent) for suspension cells?

Hello, Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?

20 February 2023 2,293 0 View

How to improve the concentration of input samples during immunoprecipitation?

Hello, I am doing co-immunoprecipitation to study the interaction of my protein of interest. The protein concentration after performing the Bradford assay for input samples is deficient. Does...

21 October 2022 2,309 8 View