I am about to perform RNAseq on E. coli total transcriptome.
I am planning to use the Nextera kit so I need to prepare my own cDNA from total RNA.
Since the kits for ds cDNA synthesis are crazily expensive, I am planning to use a Tetro cDNA synthesis kit for first strand synthesis with random examers and then use a second stran buffer + polI+ all the components to synthesize the second strand.
Do you have experience on this?
Could this work fine?