Francesca Ceroni

4 Questions 8 Answers 0 Followers

Questions related from Francesca Ceroni

Do you know the protocol for optimizing fragment size with random primers?

Does anybody have or know a protocol to understand how to optimize RNA/random hexames/temperature in ds cDNA synthesis reaction to get a precise fragment size?

21 January 2014 8,136 2 View

Does anybody know which is the size range of fragments yielded by a first strand cDNA synthesis reaction starting from RNA?

Is there any way to control it?

17 January 2014 3,593 5 View

Single read vs paired end sequencing in RNAseq?

Which is the advantage of performing a paired end sequencing instead of a single read one? I know it gives information for eukaryotic samples about the splicing variants... but for prokaryotes?

17 January 2014 3,451 5 View

ds cDNA synthesis for RNAseq?

I am about to perform RNAseq on E. coli total transcriptome. I am planning to use the Nextera kit so I need to prepare my own cDNA from total RNA. Since the kits for ds cDNA synthesis are crazily...

16 January 2014 7,579 13 View