Francesca Ceroni

6 Questions 9 Answers 0 Followers

Questions related from Francesca Ceroni

Can anyone provide feedback on traces from DS cDNA libraries on Bioanalyzer?

I have run my DS cDNA libraries on the Bioanalyzer [see the traces attached]. There is a weird small peak near the lower marker peak and I do not know if these libraries are good or not. Can...

26 June 2014 9,362 3 View

Ds cDNA analysis after retrotranscription?

I did a two step ds cDNA synthesis starting from 50 ng and 150 ng enriched mRNA. How can I check my cDNA? I do not see peaks at Bioanalyzer, and I am wondering if the material is too low or if it...

13 March 2014 9,662 2 View

Do you know the protocol for optimizing fragment size with random primers?

Does anybody have or know a protocol to understand how to optimize RNA/random hexames/temperature in ds cDNA synthesis reaction to get a precise fragment size?

21 January 2014 8,187 2 View

Does anybody know which is the size range of fragments yielded by a first strand cDNA synthesis reaction starting from RNA?

Is there any way to control it?

17 January 2014 3,639 5 View

Single read vs paired end sequencing in RNAseq?

Which is the advantage of performing a paired end sequencing instead of a single read one? I know it gives information for eukaryotic samples about the splicing variants... but for prokaryotes?

17 January 2014 3,490 5 View

ds cDNA synthesis for RNAseq?

I am about to perform RNAseq on E. coli total transcriptome. I am planning to use the Nextera kit so I need to prepare my own cDNA from total RNA. Since the kits for ds cDNA synthesis are crazily...

16 January 2014 7,652 13 View