Hi,
I wanted to know about the drug release behaviour under HPLC method.
I am trying to get the peak for release profile of nanoparticle-Paclitaxel drug in 1X PBS for different time points whereas I got the peak for loading. Nanoparticle is calcium phosphate. Could anyone suggest the possible solution for the same.
Hi,
Have a look at these papers:
Eur J Pharm Biopharm. Author manuscript; available in PMC 2016 May 1.
Published in final edited form as:
Eur J Pharm Biopharm. 2015 May; 92: 120–129.
Published online 2015 Mar 7. doi: 10.1016/j.ejpb.2015.02.017
PMCID: PMC4430429
NIHMSID: NIHMS670633
Release Kinetics of Paclitaxel and Cisplatin from Two and Three Layered Gold Nanoparticles
Christopher G. England, et al.
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Mol Pharm. Author manuscript; available in PMC 2016 Mar 2.
Published in final edited form as:
Mol Pharm. 2015 Mar 2; 12(3): 997–1003.
Published online 2015 Feb 17. doi: 10.1021/mp500817h
PMCID: PMC4764376
NIHMSID: NIHMS759617
Release Kinetics Study of Poorly Water-Soluble Drugs from Nanoparticles: Are We Doing It Right?
Sara A. Abouelmagd, et al.
---------------------
Paclitaxel Drug Delivery from Cardiovascular Stent - medIND
medind.nic.in/taa/t06/i1/taat06i1p88.pdf
by D Kothwala
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Hi Ashu,
I encourage you to follow dialysis method using dialysis tubing (available commercially) of MWCO 5000 Dalton. You can intermittently sample the release media and analyze it using HPLC Method.
Wishes
Rakesh
Hi Ashu,
Yes, I suggest you to get a proper method for detecting Paclitaxel by HPLC. And, choose appropriate simulated media to test release studies by dialysis method and perform the release study. The collected samples over time can be freeze-dried, and dissolve in appropriate solvent before quantifying with HPLC.
All the best,
Niranjan Kotla
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