Could you recommend a Journal to publish a literature review about the relationship between globalization and ultranationalism in Europe?

Currently I am finishing the literature review about the relationship between globalization and the resurgence of ultranationalism parties in Western Europe. This is the first part of my Doctoral...

02 October 2020 9,773 5 View

Cordial saludo, necesito retirar nombre de la imagen diagnostica de mamografia con Python com lo hago?

en la imagen diagnostica aparece el nombre de cada imagen CCD, CCI deseo borrar la imagen del nombre alguien me puede ayudar agradezco su ayuda , gracias

11 May 2020 9,205 2 View

How to reply to a DebutyEditor for more explanations, after refusing your Manuscript?

The DebutyEditor from a Journal refused directly our Manuscript after peer-review process. In the refusal letter, only the third reviewer is attached, with general comments. How can we ask for...

07 February 2020 862 2 View

The centre of special real jordan algebra when is equal to its commutant?

The question is simple: Let A be associative real algebra and A(+) the related jordan algebra with product A * B= 1/2(AB+BA). The centre of A(+) denoted by z(A(+) ) (set of ''operator...

28 December 2019 3,647 5 View

Can you grow HEK293 FreeStyle on plate (rather than suspension)?

Hi RG community, I want to use HEK293 FreeStyle cells to express an endogenous edited protein. They are optimized to grow in suspension; however, for clone screening purposes, I would like to...

01 July 2019 2,033 5 View

How do you do a qualitative research?

I am in the journey of learning to do qualitative research and I want to learn on how to do it.

16 June 2019 4,863 9 View

Are there any guidelines for descriptive review of a biomolecule of interest? Can you link me any already published works about?

I would like to perform a systematic review about a specific biomolecule and its role in cancer. I want to follow a protocol, like PRISMA and Cochrane's handbook for systematic review and...

25 January 2019 8,765 3 View

How to represent a MATRIX27 or MASS21 term in Ansys Workbench?

Greetings dear collegues, my question deals with representation of "directional" mass and damping in ANSYS Worbench. In detail, I need to represent the added mass and the radiation damping of an...

25 October 2018 1,867 3 View

How do you add a volumetric heat flux in Abaqus?

I wonder if you are able to assist me with the DFLUX subroutine in Abaqus. Thus far I have been able to simulate the moving surface heat flux from a laser beam with a Gaussian distribution over...

30 November 2017 5,412 3 View

Is somebody currently investigating the "Ideal GF" phenomena in the internent/social media?

Ideal GF is a new kind of meme, I guess, where the drawing of a girl is sorrounded with text, creating the profile of an ideal girlfriend. I wonder if there are reseachers interested in this topic.

06 November 2017 5,293 1 View

Is there any pipeline for 16s metagenome bacterial classification based on their functions?

In 16s rDNA metagenome analysis, after sequencing data. I want to classify bacteria based on their functions. I am looking for a Pipeline or any software that can do this job?

13 January 2021 533 6 View

No host reference for metatranscriptomics?

One of the steps during the preprocessing of the data from metatranscriptomic analysis is to remove any host reads (host contamination) by comparing to host database. But what if there is no host...

30 November 2020 3,259 2 View

What the best way to improve Illumina genomic assembly with nanopore metagenomic data?

I have the Illumina reads and assembly of my bacteria from pure culture DNA sequencing and the nanopore sequencing data from the metagenome (DNA of co-culture). Does anyone know the best way to...

14 July 2020 6,516 3 View

Any recomendations for selective lysis of Eukaryotic cells stored in RNAlater or Ethanol?

Hello everybody, --just a brief intro about the background of my question-- I have to analyse the gut microbiome of a marine fish through whole metagenome sequencing. Going through literature, I...

18 February 2020 8,460 1 View

Total DNA Quality for 16S Amplicon Sequencing ?

Dear all, I performed DNA extraction using Qiagen DNeasy Blood and Tissue Kit, followed the protocol with an addition of 2ul RNase A with incubation of 2 min at R.T after adding proteinase K....

01 February 2020 5,395 4 View

Preservation of freshwater samples (in sterivex filter) using dry ice+ 100% ethanol bath for subsequent metatranscriptomics analysis?

17 December 2019 9,172 2 View

Does genomic DNA bands smear on gel are degraded DNA?

I have to send my sample for metagenomics sequencing but my genomic DNA shows up a clear band with thin and long smear. It looks like my sample has degraded but I'm not sure. I read somewhere that...

15 December 2019 206 3 View

How to perform differential gene expression analysis for a DESeq2 dataset with multiple factor levels?

I'm a novice to RNA-seq analysis and DGE, hoping to get some good ideas moving forward. For context: I have 15 samples, 3 replicates each of 5 sample types. They're mRNA samples obtained from...

09 October 2019 8,206 4 View

How to extract good quality total DNA from insect larvae for metagenomic purpose?

Good day, currently I am practicing extracting total DNA using drosophila melanogaster (fruit fly) adult as well as larvae as my sample with Qiagen DNeasy Blood and Tissue Kit. However, I keep...

28 August 2019 601 3 View

How much i should add proteinaseK for RNA extraction and how to use it?

I have problem with RNA extraction from soil. My RNA have low yield and purity so i want to give a proteinaseK treatment to the reaction. How much i should add proteinaseK and how to use it? Thank you

07 August 2019 3,177 3 View