Carlo Breda

6 Questions 6 Answers 0 Followers

Questions related from Carlo Breda

Drosophila Fmr1 Delta 50.... will they be homozygote?

Hey all, is someone using Drosophila files carrying a mutation in the fmr1 gene in particular the Delta50. All my stock are heterozygotes for the mutation being Tb... what do researcher for...

16 January 2025 4,275 0 View

RNA-seq Drosophila. Recommended number of Reads?

Hi Guys, I would like to do an experiment where I want to investigate the level of differently expressed genes in different Drosophila melanogaster genotypes by using RNA-seq. Does anyone have an...

07 April 2017 827 1 View

Does anyone have a protocol for extraacting proteins from Drosophila dissected entire brains?

I am planning to do some WB from Drosophila heads. Because the major component of heads are the eyes, I would like to do WB only from brains by dissecting them. Does anyone have an idea how many...

18 November 2016 8,907 1 View

Does someone have a nice detailed protocol for extracting transporter from the membrane for Western Blot?

Hi Guys, I need a detailed protocol for trying to extract an overexpressed transporter for HEK293T cells [from the plasma membrane]. So far I did use the NP-40 buffer but I guess with very bad...

16 June 2016 5,812 1 View

Can rat tail callagen II be reused?

Hi, I need to coat some plate with rat tail collagen II. I was wondering whether I can reuse the collagen after I do the first coating.... if so, how many times? Thank you in advance

06 May 2016 4,350 3 View

Software to recording Drosophila behaviour using a USB webcam

Dear all, I would like to make some Drosophila behaviour recording using an USB webcam. Could you suggest a program (possibly a freeware) which will allow me to: -change the fps of my recording -...

01 January 1970 748 4 View