DREADDs targeting at cholinergic cells- any suggestions or experiences?

06 May 2016 1 5K Report

We are considering to use the DREADDs targeted towards the cholinergic cells to silence them and was wondering if people would like to share their thoughts/experience?

Simon C Cork

I don't have any experience of using DREADDs in cholinergic cells, but have used them in other neuronal populations. My advice would be to test them in vitro first (patch clamp or Ca2+ sensing) to make sure that the receptors are fully functional before performing whole-animal experiments

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