The DPPH (2,2-diphenyl-1-picrylhydrazyl) assay is a common method used to evaluate the antioxidant activity of a sample. It measures the ability of antioxidants to donate hydrogen atoms or electrons to neutralize free radicals, which can be indicators of the sample's antioxidant capacity.

How the DPPH Assay Works:

Steps in the DPPH Assay:

Expression of Results:

The results are typically expressed as a percentage of DPPH radical scavenging activity or as an IC50 value (the concentration required to reduce the initial DPPH concentration by 50%).

The DPPH assay is widely used due to its simplicity, speed, and ability to screen the antioxidant potential of various natural extracts, food products, and pure compounds.