I need to do Double In situ Hybridization , is anyone having optimized protocol for it , Please help me with this , we have been doing In situ hybridization in our lab using DIG labelled probe with NBT/BCIP and it worked well, but what can i use for labelling other probe and to develop it what should i use other than NBT/BCIP???
Thankyou Milica:), i tried similar way but with Fluo labelling instead but i have problem while detection with INT/BCIP..i already have Fluo labelled probes but dont want to go for FISH or any flourescent detection, give me suggestion for more detection ways other than NBT & INT for AP
Also what i found strange is as soon as i add INT to NTMT it precipitates to crystals does anyone had similar problem and sorted it out
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