Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Temperature/voltage simulations of a protein?

Dear all. Is there a way to simulate temperature or voltage treatments to a single protein? If so, is there an online web server to conduct it or using BioVia Discovery/AutoDock tools? Thanks in...

14 June 2020 1,168 3 View

Which should be the concentration of extracts to evaluate in normal colon cells?

Dear all, we are evaluating the cytotoxic effect of plant extracts on HT-29 and HCT116 colon cancer cells. We have determined the IC50 values for each cell line, however, we want also to test...

09 October 2019 1,259 3 View

Flow cytometry in TryPLE detached macrophages?

Hello, I'll perform flow cytometry (Caspase-1, Annexin-V, PI assay) in differentiated macrophages (from THP-1 monocytes to macrophages using PMA). I usually detach the cells using TryPLE and wait...

04 May 2019 8,746 2 View

How to dissolve reagent grade Quercetin into cell culture medium?

Hi, I am trying to dissolve pure reagent grade Quercetin into cell culture medium (DMEM+sodium bicarbonate+antibiotics) but it clearly does not form a solution. I've found that DMSO might serve...

22 July 2018 3,847 3 View

What level of objectivity can the development of prospective thinking have based on expert judgment?

I am developing an analysis on the different ways of approaching foresight in the field of social sciences

18 June 2018 2,142 3 View

HPLC grade reagents for UHPLC-MS ?

Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance

03 February 2018 1,712 7 View

HPLC grade reagents for UHPLC-MS ?

Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance

01 February 2018 5,909 10 View

Detaching RAW 264.7 Macrophages using Trypsin?

Dear all...I'm evaluating inflammation on macrophages (cell line: RAW 264.7) but actually I'm just culturing them. Is it possible to detach them using trypsin? I've read in some articles that...

27 May 2016 6,592 3 View

Detaching RAW 264.7 Macrophages using Trypsin?

Dear all...I'm evaluating inflammation on macrophages (cell line: RAW 264.7) but actually I'm just culturing them. Is it possible to detach them using trypsin? I've read in some articles that...

04 May 2016 8,640 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View