Dot blot membrane preparation?

21 April 2016 1 3K Report

I would like to do a dot blot but am not sure about how I should prepare the membrane. Every protocol I have just says to have a membrane ready to blot with proteins. I have a PVDF membrane. Do I have to activate the membrane first with methanol before blotting?

Alexander Kollau

As PVDF membranes are hydrophobic you actually have to activate them first in methanol. Check the following link for a short protocol: http://www.agrisera.com/en/info/dot-blot-method-description.html

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