Dear friends,
I could set up micronucleus assay on cell line. but I have a big problem in positive control. I tried vast ranges of H2O2 doses for possitive control(even I used the standard data obtained from MTT assay) but in some dose I couldn't find micronuclei and in other doses the H2o2 killed the cell...I am really confused now..may I ask you give me a guide or advice to solve my problem?