I need to ask if it is possible to detect quorum sensing using real time PCR?
At other meaning, to check this phenomenon dose a researcher need to detect the bacterium causing it or there is other concept?
Some researchers says that "The DNA sequences of the dark genes are very rich in G and C molecules, two of the four bases molecules that make DNA. We need to fully understand the concept of dark...
01 February 2019 5,027 6 View
There are a big mystery in the world of molecular biology regarding the part of genome which must be present but cannot be found.
01 February 2019 9,251 11 View
Biofilm is a structure built by a community of bacteria. When the density of one type of bacteria is high, all the other bacteria sense that. At a certain point in the population density of those...
11 December 2018 1,294 1 View
Sequencing of human genome at the center of interest in the biomedical field over the past several decades and is now leading toward an era of personalized medicine. During this time, DNA...
10 November 2018 3,166 3 View
I have high quality published research, but i have low level of citations in comparing with the number of researches and their quality. How ti increase my citation as a result for reading the...
09 October 2018 9,524 2 View
In many world universities ranking like Times Higher Education, QS, and Shanghai, the researches no. is a corner stone in each one especially the first one. Many scientists are going to write a...
09 October 2018 8,154 0 View
DNA profiling is the process of determining an individual's DNA characteristics, which are as unique as fingerprints. DNA analysis intended to identify a species, rather than an individual, is...
08 September 2018 9,717 3 View
Probiotics are good bacteria that are either the same as or very similar to the bacteria that are already in your body. Your lower digestive tract alone teems with a complex and diverse community...
07 August 2018 7,380 8 View
CRISPR/Cas9 is a system found in bacteria and involved in immune defence. Bacteria use CRISPR/Cas9 to cut up the DNA of invading bacterial viruses that might otherwise kill them. Today we’ve...
07 August 2018 6,859 8 View
Which h is more preferred to detect concentration or purity of DNA? On the other hand, Does we need to detect quantification of Nucleic acid when we used automated DNA extraction UNIT like...
07 August 2018 1,615 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View