Dose anybody know how many cell i need seed in t25 flask for detecting dna laddering after 72h treatment?

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Guodong Fu

It depends on the treatment too. I would suggest to try different cell amounts like 0.3M,0.5M or 0.8M cells per flask.

Shazib Pervaiz

Seed 0.5M cells the night before and you will have enough cells to extract DNA for laddering. Good luck!

Sreedhar V

1X106 cells are seeded in T-25 flask for detecting dna laddering