Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Picrosirius red staining without picric acid?

I would like to perform picrosirius red staining but we cannot use picric acid anymore. Do anyone have already test other acid like citric acid instead? Thanks a lot

08 September 2020 2,794 2 View

Experience with siRNA transfection of neural stem cells?

Does anyone have experience in gene silencing through siRNA transfection specifically in neural stem cells (ReN cells)? I know these are hard to transfect cells, and with other methods I normally...

29 March 2020 4,210 0 View

Can anybody help me with Boyden chamber invasion assay for HCT-116 cell lines?

I have a problems with Boyden chamber assay for HCT-116 cell lines, I did not see any stained cell in the membrane. I have had a success with other cell line, A549. Does anyone have any...

23 March 2020 7,119 5 View

How to make cell distribute evenly in 12-well plate?

I worked with cell culture 1 year ago, nearly I have a problem when I seeding in 12-well plate, more cells settles in the middle of well. I am trying with different 'shaking method'. I hope to get...

19 March 2020 9,999 3 View

Can anyone provide the Scoring algorithm for the UK SF-12 Version 1?

24 February 2020 1,653 2 View

Can anyone recommend any strong papers concerning the prevalence of PTSD and/or CPTSD in paramedics?

Dear all, I am in the early stages of undertaking research surrounding PTSD/CPTSD and the prevalence of these conditions in paramedics. Can anyone point me towards some recent research in this...

29 October 2019 8,117 1 View

What is the best way to isolate the full leukocyte fraction from mouse blood?

I would like both monocytes, lymfocytes and granulocytes to eventually stain and stick on a slide. I used lymphoprep before, but this only gives me the mononuclear cells. I would like the complete...

08 October 2019 1,717 3 View

Could someone guide me towards my hesitation to choose my tax law thesis topic?

Good afternoon from Peru! I'm currently working on my thesis (Tax Law field), but I can't seem to decide which topic I would like to explore, because I am really interested in this three...

20 June 2019 4,419 4 View

Why is my whole western blot gray?

I have attached a picture for my most recent western blot. My Beta-actin always shows up completely perfect but all other protein blots appear gray and messy. This has happened multiple times....

31 May 2019 5,364 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View