Dominant and codominant marker with reference to PCR primer?

More Kamesh Krishnamoorthy Kalyankumar's questions See All

What does an "Error 111" mean on an AKTA Explorer?

Dear Colleagues, I would be grateful if any of you can advise me on what an "error 111" is on an AKTA Explorer. The manual suggests that the instrument be turned off and turned on. That does not...

13 June 2024 2,961 1 View

Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...

10 December 2023 5,680 2 View

What dilutions of serum sample should I use in ELISA?

I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...

18 February 2022 10,013 2 View

Sequence identity matrix?

While generating a sequence identity matrix in bioedit software by pasting the sequences from notepad, often there is an overlapping of sequence names (given before the fasta sequence) and the...

10 January 2020 1,339 0 View

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,151 5 View

Is the Zero Shot Learning a good current research area or not?

I am going to do my research on Zero Shot Learning. Is it good area in recent trends...

03 December 2018 7,235 3 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

14 April 2018 6,544 2 View

First aid measure for Ethidium bromide spill?

If there is an accidental ethidium bromide spill on skin/hands during a moelcular work what is the immediate first aid measure to be taken using very simple chemicals available in the lab?

12 March 2018 443 3 View

Any simple protocol for whitefly DNA extraction?

Iam finding it hard to get amplification post whitefly DNA extraction using lysis buffer method. Any other simple methodology. What are the precautions or troubleshooting measures to be followed?...

09 March 2018 3,232 4 View

Insects for DNA extraction stored in ethanol post collection?

Generally insects like whiteflies collected for DNA extraction are stored in ethanol. Does ethanol act as a preservant. Does it help in maintaining the integrity of the insect DNA. How long till...

17 February 2018 667 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Rameez Rather

dominant markers like RAPD cannot differentiate homozygous condition from heterozygous condition while co dominant marker like SSR easily distinguishes homozygotes from heterozygotes.

Mostafa Haghpanah

I agree with Rather

This link helpful

https://www.slideshare.net/mobile/FAOoftheUN/molecular-markers-types-and-applications-55688001

Mark Farman

PCR can produce co-dominant markers if the two alleles have different lengths