If we have to complement a vir gene in the Agrobacterium mutant, is it necessary to complement the vir gene promoter too?
I am planning to use pBBR1MCS-5 vector which has Lac/lac, T3, T7 promoters, are any of these promoters sufficient for my vir genes to be expressed in Agrobacterium mutant?.
Thank you in advance
It think the most important factor is whether the vir genes are tightly regulated and you want your complementing vir gene to be expressed similarly to other genes in the pathway. You could include your vir gene promoter in your clone to get around this.
However if regulation is not critical then I think the lac promoter will be fine. You don't need (and probably don't want) the super high level expression of T3 or T7 promoters since that would not be physiologically similar.
Thank you Professor, I will go ahead with lac promoter as the regulation is not very critical and we just want to see the qualitative functional complementation. Thank you for your time. Regards.
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