Veeresh Lokesh

3 Questions 3 Answers 0 Followers

Questions related from Veeresh Lokesh

Why am I seeing expression of a gene which is mutated by transposon insertion in Agrobacterium?

Hello all, we are working on functional validation of a transposon mutated virulence genes in Agrobacterium tumefaciens. However, in the mutant, when the gene expression is analyzed by qPCR, we...

21 November 2024 6,489 3 View

Does the Vir gene complementation in Agrobacterium mutants requires the expression of promoters too?

If we have to complement a vir gene in the Agrobacterium mutant, is it necessary to complement the vir gene promoter too? I am planning to use pBBR1MCS-5 vector which has Lac/lac, T3, T7...

08 February 2024 5,496 2 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

11 February 2021 2,993 3 View