saccharification is the process of breaking down complex carbohydrates, such as starch, into simple sugars, such as glucose, by the action of enzymes 1. The saccharification yield is the percentage of glucose obtained from the total amount of starch in the biomass 2.

One possible formula for calculating the saccharification yield is:

Saccharification yield (%)=Glucose concentration (g/L)×Hydrolysate volume (L)Starch concentration (g/L)×Biomass weight (g)×100Saccharification yield (%)=Starch concentration (g/L)×Biomass weight (g)Glucose concentration (g/L)×Hydrolysate volume (L)​×100

However, the saccharification yield may vary depending on the type and pretreatment of the biomass, the enzyme loading and activity, the pH and temperature of the reaction, and the duration of the hydrolysis 34. Therefore, it is important to optimize these factors to achieve high saccharification yields.