I'm using petunia for protoplast extraction and gene transformation.
It's been subcultured many many times and I guess the plants have been used for more than
3-4 years.
We find it difficult to have the colonies form calli these days.
One says maybe it's because the plants are old.
But our petunia seems to produce new buds well with each subculture.
Does the total age of the plants really affect the vigor and viability to regenerate?
That's a very interesting issue. It does matter with regard to vitality but vary as per different plant species. You need to standardise this issue..but quite a possibility....
We have seen the same with many PGPMs..
The production of shoots or plantlets from callus depends on several factors, including:
1. Genetic factor according to plant type and variety.
2. The age of the plant from which the explant is taken.
3. The type of explants such as leaf, bud, root and shoot tip.
4. The type and concentration of the growth regulator that is added to the media.
5. The type and concentration of media used for accurate reproduction.
Thank you and good luck.
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