In the field of microbiome metagenomics, I would preferably want all the bacteria within my sample to be sufficiently lysed, thus aiming for an all-in-one or one size fits all lysis protocol. From all the literature, I can surely say that I would need at least a few lysing methods.

More specifically, I would like to know if there are any minor tips and tricks (published/unpublished) in what order these steps come into play. Of course, there are cases in which some steps cannot precede others but there are a lot of combinations.

For instance, I incubated cell pellets with lysozyme and then treated them with ultrasound, which yielded significantly higher DNA output, compared to both methods alone. How about vice-versa? Are there any other combinations coming to mind that might be better off swapped?