Why SEM Image shows Cracks After Gold Sputtering?

I am trying to assess the dispersion of MWCNT Oil nanofluid. I dry my sample for 1 day in the furnace on an aluminum foil. Then I stick this foil on double-sided carbon tape. The image quality was...

11 December 2019 4,904 6 View

DSC for heat capacity of MWCNT vegetable based oil ?

I am new to using DSC and I am trying to use it to measure the heat capacity of nanofluid with the classical three steps procedures. And since there is no clear guidance regarding the ramp rate...

10 November 2019 5,913 4 View

How to control moisture in sugar coated vermicelli?

what are the parameters to control the moisture in sugar coated vermicelli?

07 May 2019 6,962 2 View

Does anyone know how can I submit a manuscript to "Dairy Science and Technology"?

the link they provided by their website does not work http://editorialmanager.com/dste

03 April 2018 9,628 6 View

Which ASTM series is applied to tensile testing of CNT reinforced PDMS elastomer?

Dear all, I have made a nanocomposite of CNT-PDMS nanocomposite and wish to do tensile testing of this composite. I came across papers with other material wherein they follow specific ASTM...

22 March 2018 2,314 3 View

Do we need to heat MWCNTs before making nanocomposite with polymers?

03 August 2017 9,839 5 View

I could not get conductivity when I made upto 15% Carbon black in PDMS matrix to make nanocomposite.

I am trying to make Carbon black/PDMS nanocomsite for piezoresister. However, ever after using upto 15% carbon black, I could not get conductivity. I dont walk to increase concentration further as...

22 July 2017 3,150 5 View

Which of the filler-Silver nanoparticles, Carbon Black, Graphene or CNTs would increase the young's modulus of polymer composites?

22 July 2017 9,497 5 View

What is the general procedure for extraction of sinigrin and allyl iso thiocyanate from mustard seeds and leaf?

15 March 2016 9,080 1 View

Problem with phosphoprotein detection? BSA vs Milk and TBST vs PBS??

We have been having problems with high background in our western blots for phospho-proteins even though we are using BSA. We found that certian lots of BSA give high background. What are your...

03 February 2016 4,055 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View