Mohamed Abu-farha

14 Questions 27 Answers 0 Followers

Questions related from Mohamed Abu-farha

Problem with phosphoprotein detection? BSA vs Milk and TBST vs PBS??

We have been having problems with high background in our western blots for phospho-proteins even though we are using BSA. We found that certian lots of BSA give high background. What are your...

02 February 2016 7,902 2 View

How to calculate protein concentration in a competitive ELISA?

I have done a competitive ELISA for the first time and I am not sure about the way to calculate the concentrations? I did a log transformation of the data to generate the standard curve? Is this...

01 January 2014 5,009 4 View

What is your advise for optimal long range PCR?

I am trying to amplify a large fragment of about 12k bp from genomic template. I have tried a few things and its not working using a long range kit. Any tricks that you recommend from people who...

11 November 2013 8,406 9 View

What is the best cell line for glucose uptake assay?

I have been trying to do a glucose uptake experiment using fluorescent dye to measure if my protein of interest will have impact on glucose uptake. From experience, what is the best cell line for...

11 November 2013 4,927 24 View

Does anyone have recommendation for THP1 transfection?

THP1 transfection

09 September 2013 3,094 6 View

Does anyone have Orbitrap Fusion?

The Thermo ScientificTM Orbitrap FusionTM TribridTM mass spectrometer combines the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture that delivers...

09 September 2013 10,040 2 View

Does anyone have experience with the Lipofectamine 2000 toxicity?

Do you have a toxicity problem with lipofectamine 2000? wht type of cells and what do yuo do to solve it?

09 September 2013 8,955 12 View

Does anyone have experience with using DNA synthesis for cloning?

I was interested in using synthesized DNA for cloning. I am still used to the PCR based cloning. Any suggestions or recomendations before starting this process? Any good companies?

09 September 2013 4,695 12 View

What is the difference between polymorphism and a mutation?

I got a little confused about the difference between a mutation and polymorphism that people use in genetics.

09 September 2013 7,792 89 View

Which one is better for gene expression quantification, Branched DNA or Real time PCR?

In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to detect nucleic acid molecules. I have been asked about which is...

09 September 2013 8,450 46 View

Have you compared real time PCR expression data to western blot protein expression data?

How much correlation would you expect between real time PCR and western blot data?

09 September 2013 9,795 8 View

Does anyone know if there is a commercial source for SuperSILAC protein mix?

I was wondering if there is a commercial source for super silac protein mix that are digested and ready made for use in MS.

09 September 2013 2,716 2 View

Does the function of the protein affect the correlation between its gene and protein expression?

09 September 2013 9,847 5 View

Any recommendations for 3T3L1 differentiation?

I am trying to differentiate 3T3L1 cells into adipocytes, any helpful suggestions?

09 September 2013 3,931 9 View