Hello!
I have some expired Ni-NTA resin (QIAGEN), and I need to purify my His-tagged protein.
Does the expiration date affect the binding capacity of Ni-NTA?
Thank you in advance
Hi,
I think it can be used even past its expiration date as long as it is kept under the right conditions, which I usually do when experimental materials are lacking. However, it is important to note that this may increase the uncertainty of the experimental operation. If the experimental result is not good, then we cannot rule out the problem of experimental materials. If the result is ideal, then do we have to use the expired materials to ensure the repeatability of data? Therefore, I recommend using fresh resin whenever possible.
Dear Mohammad
What di you mean with "after expiration date"? Is it expired from days? Months? Yiears?
However ad Robert told, if it sas correctelly stored, i do not think that there are problems. The resins is quite simple and it is function is just non è the metal therefore if during the wash with water and equilibration the color associate to che nickel binding is retained and the flow of the coloumn is ok (not unaxpected counter-pressure) i dont see any reason why it has to do not work.
Good luck
Manuele
Dear Mohammad,
in the past I worked with many Qiagen IMAC resins. When we checked retain samples even from ten years old syntheses, there were no losses in binding capacity. So the chance for good results is high, when stored properly. But, as Roger pointed out, you need to document the use of an already expired product.
Best, Roland
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