What is solution should I use to obtain nitrogen excess in hydroponics?

I am growing wheat in hydroponics and I want to perform nitrogen excess stress. Does anybody knows what should I add to Hoagland solution and what concentration to obtain the best stress?

06 July 2018 4,804 4 View

Does anybody design a PCR reaction to quantify the number of mutated gene copies, i.e. design a PCR specific only to the mutant DNA fragment?

I want to determine the level of crossing of one rapeseed variety ("A") with another variety ("B") that has a known SNP in the target gene. If it comes to a crossing, then in DNA isolated from...

27 June 2017 6,622 9 View

Does anybody design a PCR reaction to quantify the number of mutated gene copies, i.e. design a PCR specific only to the mutant DNA fragment?

I want to determine the level of crossing of one rapeseed variety ("A") with another variety ("B") that has a known SNP in the target gene. If it comes to a crossing, then in DNA isolated from...

05 June 2017 2,360 10 View

Is spectrophotometric assay of RNA quality better than electrophoretic one?

Dear all, I have problem with my electrophoretic assay of RNA quality. I performed native agarose gel and I obtained no clear result. The marks is very good, but samples look as RNA is...

04 May 2016 1,539 8 View

RNA isolation. After I added isopropanol to my sample it became cloudy. After centrifugation I saw huge white pellet. Can you help me?

What should I do to clarify my sample? Probably my samples are contaminated by polysaccharides. I washed samples with 70% ethanol many times, I centrifuged samples and precipitated again by...

01 February 2016 3,346 9 View

What is the difference between the Duncan test and Tukey test and which of them is more suitable in my case?

Which test is more suitable for plant science? I have plants which are treated with two different chemicals, so I have control plants, and two variants with above mentioned chemicals. Those plants...

26 February 2015 5,778 5 View

What is the difference between the Duncan test and Tukey test and which of them is more suitable in my case?

Which test is more suitable for plant science? I have plants which are treated with two different chemicals, so I have control plants, and two variants with above mentioned chemicals. Those plants...

01 February 2015 2,332 3 View

Does anybody know how to successfully assay the activity of malate dehydrogenase in cucumber plants?

31 December 2013 4,759 0 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View