Does anybody design a PCR reaction to quantify the number of mutated gene copies, i.e. design a PCR specific only to the mutant DNA fragment?

I want to determine the level of crossing of one rapeseed variety ("A") with another variety ("B") that has a known SNP in the target gene. If it comes to a crossing, then in DNA isolated from...

27 June 2017 6,622 9 View

Does anybody design a PCR reaction to quantify the number of mutated gene copies, i.e. design a PCR specific only to the mutant DNA fragment?

I want to determine the level of crossing of one rapeseed variety ("A") with another variety ("B") that has a known SNP in the target gene. If it comes to a crossing, then in DNA isolated from...

05 June 2017 2,360 10 View

Is spectrophotometric assay of RNA quality better than electrophoretic one?

Dear all, I have problem with my electrophoretic assay of RNA quality. I performed native agarose gel and I obtained no clear result. The marks is very good, but samples look as RNA is...

04 May 2016 1,539 8 View

RNA isolation. After I added isopropanol to my sample it became cloudy. After centrifugation I saw huge white pellet. Can you help me?

What should I do to clarify my sample? Probably my samples are contaminated by polysaccharides. I washed samples with 70% ethanol many times, I centrifuged samples and precipitated again by...

01 February 2016 3,346 9 View

What is the difference between the Duncan test and Tukey test and which of them is more suitable in my case?

Which test is more suitable for plant science? I have plants which are treated with two different chemicals, so I have control plants, and two variants with above mentioned chemicals. Those plants...

26 February 2015 5,778 5 View

What is the difference between the Duncan test and Tukey test and which of them is more suitable in my case?

Which test is more suitable for plant science? I have plants which are treated with two different chemicals, so I have control plants, and two variants with above mentioned chemicals. Those plants...

01 February 2015 2,332 3 View

Does the enzyme Ascorbate oxidase only work in apoplast in plants?

Do you know any relationship between the decrease in dehydroascorbate concentration and increase in ascorbate oxidase activity?

02 March 2014 5,710 0 View

Does anybody know how to successfully assay the activity of malate dehydrogenase in cucumber plants?

31 December 2013 4,759 0 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View