H2O2 assay in plant suspension cultures?

Joanna Chojak-Koźniewska @Joanna-Chojak-Kozniewska

07 October 2022 1 878 Report

Does anyone know if there is any non-fluorescent but acurate methos for h2o2 assay in plants suspension culture?

Thank you for help.

Hello Joanna Chojak-Koźniewska ,

Have you tried any of the following H2O2 indicators: titanic acid dissolved in sulfuric acid or Dr. Wurster’s paper (paper soaked with tetramethylparaphenylenediamine and then dried)?

Regards,

Tom Cuff

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Joanna Chojak-Koźniewska's questions See All

How to test genetic stability of bacteria?

Hi! I'm looking for a paper or a protocol of a validated method for testing of genetic stability of bacteria. Does anybody know sth like that? We were looking for the papers on this subject but we...

12 January 2021 490 8 View

Is there any easy way to check if given miRNA hybridize to RNA (extracellularly)?

Suppose we have given miRNA (that according to in silico analysis should bind to a specific sequence in RNA) that is labeled with a fluorescent dye (or is labeled otherwise) and we have isolated...

12 November 2020 5,188 1 View

Is anyone interested to join me to the Erasmus project?

I'm Erasmus coordinator from Poland. I'm open to cooperate with another coordinator within Erasmus project. If you are looking for the team, please write to me. Joanna

18 July 2020 7,910 2 View

Is anyone interested in collaborating on an article in the field of effective time management?

I'm looking for someone that is interesting collaboration in effective time management area.

15 July 2020 9,068 5 View

Has anyone done research what are the determinants of implementing industry 4.0 solutions from the point of view of PESTEL factors?

I am looking for research results, scientific articles, tips which factors affect on Industry 4.0 implementation and spread of using in practice. Maybe simple PESTEL factors could be useful -...

17 June 2020 7,494 1 View

What statistical analysis would you recommend to use for my survey data?

Hi everyone, I am currently doing my dissertation for MSc in Strategic Management. My topic focuses on the impact of social media on customer service: case study on Twitter accounts. I have...

24 May 2020 5,275 5 View

What scales could be used to measure wellbeing during the coronavirus pandemic?

I'm looking to find some outcome measures that could address how people feel they are able to cope psychologically during the pandemic and wondered if anyone had any thoughts? Maybe something...

30 April 2020 8,316 3 View

Do I need to address temporal autocorrelation when using boosted regression trees?

I am using boosted regression trees (BRT) to investigate the environmental influences of shark presence at a single location. I have a year-long dataset of acoustic tag detections and...

28 April 2020 914 4 View

Could you help with a survey that concentrates on contact lens behaviours and opinions during COVID-19 pandemy?

Please see the attached invitation to the survey.

25 April 2020 2,635 3 View

What is the possible reason why MM calculation of a protein in Spartan suddenly stopped computing?

I am currently running MM (MMFF94) of a protein-ligand complex for geometry optimization using Spartan '18. The calculation went smoothly until the 19919th cycle when it suddenly stopped computing...

19 March 2020 3,628 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View