Joanna Chojak-Koźniewska

7 Questions 11 Answers 0 Followers

Questions related from Joanna Chojak-Koźniewska

What is solution should I use to obtain nitrogen excess in hydroponics?

I am growing wheat in hydroponics and I want to perform nitrogen excess stress. Does anybody knows what should I add to Hoagland solution and what concentration to obtain the best stress?

07 July 2018 4,861 4 View

Does anybody design a PCR reaction to quantify the number of mutated gene copies, i.e. design a PCR specific only to the mutant DNA fragment?

I want to determine the level of crossing of one rapeseed variety ("A") with another variety ("B") that has a known SNP in the target gene. If it comes to a crossing, then in DNA isolated from...

06 June 2017 2,406 10 View

Is spectrophotometric assay of RNA quality better than electrophoretic one?

Dear all, I have problem with my electrophoretic assay of RNA quality. I performed native agarose gel and I obtained no clear result. The marks is very good, but samples look as RNA is...

05 May 2016 1,592 8 View

RNA isolation. After I added isopropanol to my sample it became cloudy. After centrifugation I saw huge white pellet. Can you help me?

What should I do to clarify my sample? Probably my samples are contaminated by polysaccharides. I washed samples with 70% ethanol many times, I centrifuged samples and precipitated again by...

02 February 2016 3,428 9 View

What is the difference between the Duncan test and Tukey test and which of them is more suitable in my case?

Which test is more suitable for plant science? I have plants which are treated with two different chemicals, so I have control plants, and two variants with above mentioned chemicals. Those plants...

02 February 2015 2,417 3 View

Does the enzyme Ascorbate oxidase only work in apoplast in plants?

Do you know any relationship between the decrease in dehydroascorbate concentration and increase in ascorbate oxidase activity?

03 March 2014 5,756 0 View

Does anybody know how to successfully assay the activity of malate dehydrogenase in cucumber plants?

I've tried many times, but unsuccessfully. I performed homogenisation in 50mM Tris buffer pH 7,6, which contained 1mM EDTA, 1mM MgCl2, 10mM β-merkaptoetanol, 1mM DTT, 0,5% PVP. Also, I used PMSF...

01 January 2014 4,801 0 View