Ideal samples packaging for edible oils?

Hello everyone, I have maybe a stupid question regarding the right packaging to use when comes to send some oil samples in external labs. Do you have any advice about that? Is there a risk that...

18 January 2021 7,826 4 View

Alternative to use of ethanol in extraction process?

Dear all., I am using ethanol as part of the extraction process of Chlorella vulgaris carbohydrates. Since I am trying to scale up a bit the whole process, I should use much more ethanol, that in...

27 August 2020 1,875 5 View

Do I have to activate NR8383 rat macrophages with LPS before incubation with particles and investigation of cytokines release?

Hello everybody, I'm planning to investigate the in-vitro release of different markers (CXCL10, INF-a, IFN-b, 1L-1a, IL-1b, IL-6, free DNA) by NR8383 rat macrophages, upon incubation with several...

20 August 2020 8,791 1 View

Do you know if Covid-19 dataset is available somewhere?

I'm searching for a numerical dataset about the virus. I found only daily statistical data but i would like access to single patients data. Does anyone know about it?

09 March 2020 6,626 85 View

How do I report stats for a zero inflated negative binomial model?

I need to report my statistical findings in a paper but I am struggling to report the output from R. how do I treat the two sections (Count model coefficients and Zero Inflation model...

28 January 2020 1,422 2 View

What statistical method would you use to analyse point count data on butterfly migration?

I have a set of data to analyse on butterfly migration. Unfortunately my stats skills are not as good as my field skills! Me and my peers have counted the amount of butterflies migrating in one...

09 January 2020 8,150 11 View

How to choose a suitable journal?

Dear researchers, I know some sites that can help to choose a suitable journal to a given manuscript, namely: journalfinder.elsevier.com journalsuggester.springer.com journalfinder.whiley.com can...

13 October 2019 1,453 3 View

Buffer without monovalent salts, can I use Tris?

18 April 2018 8,432 1 View

Alternative to PBS buffer with low salt concentrations?

Hello all, I find myself in need to perform serial dilution of E.coli cells for a luminometric ATP assay. Normally for serial dilution I would use PBS, but from what I understood the...

18 April 2018 5,359 3 View

Toxicity of H2O2 against coli?

Greetings, I am currently trying to determine the toxicity of the effluents of an Electro-Fenton reactor with macrotoxicity method and colony count. The concentrations of H2O2 I calculated...

13 January 2018 1,660 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View