I have a mix of cd8+ and cd4+ tumor infiltrating t-cells isolated from patient tumor. I am expanding them in vitro in media supplemented with IL-2 at a concentration 5U/ml. I believe that IL-2 supplementation helps in the survival and expansion of T-cells and not activation to cytotoxic Tcells. When i added them to a HLA matched 3D cancer culture, with a 3xhigher IL2 dose (as i did not want to change medium) i found that the cancer cells died. Could it be that the Tcells were activated due to higher IL-2? Or could it be because the Tcell were already active as these are tumor infiltrating lyphocytes that were already primed with the tumor antigen?
Here's an article that may be helpful.
Article Role of IL-2 in cancer immunotherapy
Look at the following links
http://www.jimmunol.org/content/135/4/2865.short
http://jem.rupress.org/content/201/5/723.short
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